The State Department said: "The regime’s lack of compliance with the IAEA on safeguards obligations has led to the agency’s loss of 'continuity of knowledge.'"
The United States is offering a reward of up to $20 million as well as "possible relocation" for information on "Iran’s nuclear program," according to the US military.
The State Department's Rewards for Justice initiative is offering the reward to people who can provide knowledge of its "facilities, equipment, and associated personnel, such as those in the scientific community, or the movement of any of the above," US Central Command said.
The Rewards for Justice programme was established in 1984 to offer money for information that could help protect national security.
In a statement on the reward, the State Department said: "The regime’s lack of compliance with the IAEA on safeguards obligations has led to the agency’s loss of 'continuity of knowledge' regarding Iran’s production and inventory of key components like centrifuges."
President Donald Trump has repeatedly said that US forces have "obliterated" Iran’s nuclear facilities.
"We're also ensuring that Iran will never have a nuclear weapon," Trump said on Friday during a speech at the Kennedy Space Center in Florida. "They will never have it. Not even close. They're not close."
"If they did have it, if we didn't go in with those beautiful B2 bombers a little more than a year ago, if I allowed them to have it today, there would be no Israel," he added.
Tehran's nuclear ambitions have been central to negotiations over ending the Iran war, which began in late February following US and Israeli strikes on the country.
Trump's comments came as Iranian President Masoud Pezeshkian confirmed that his government was continuing efforts to resolve the conflict through dialogue.