The 46-year-old Gülşen -- often labelled as Turkish Madonna -- made the allegedly hateful quip on stage in April, but the video of the incident only became popular on social media in recent months.
A Turkish pop star could face up to three years in prison after she was indicted Friday for "inciting hatred and enmity" over a joke she made about religious schools in the country.
Singer-songwriter Gülşen -- often labelled as Turkey's Madonna -- was jailed last week but was released four days later and placed under house arrest.
The charges stem from a quip the singer made during a concert in April when she joked that one of her musicians' "perversion" came from attending a religious school. A video of the singer's comment began circulating on social media recently, with a hashtag calling for her arrest.
Turkish President Recep Tayyip Erdoğan and many members of his Islam-based ruling party are graduates of religious schools called Imam Hatip, which were originally established to train imams.
The 48-page indictment has 702 complainants, including individuals, a pro-government women's rights organization and an association from the religious school.
Turkey's penal code criminalises incitement of hatred and enmity against different groups in society based on class, race, religion or sect, requiring a prison sentence in cases that lead to threats against public safety.
In her statement to the police included in the indictment, Gülşen said she had joked with a band member whose nickname was "imam" but who had not attended the school.
"I never said this to insult or denigrate students of Imam Hatips or a segment of our country. My respect for all values and sensitivities of the country is eternal," she said and denied the charges.
The 46-year-old singer had previously become a target in Islamic circles due to her revealing stage outfits and for unfurling an LGBTQ+ flag at a concert.
A court has to approve the indictment for the trial to begin. A request by Gülşen's lawyer for her to be released from house arrest was rejected.