Israeli troops have clashed with Palestinian protesters at Qalandiya checkpoint near Ramallah on the West bank.
At least 16 people were killed and dozens injured on the day Palestinians call Nakba or catastrophe and mourn the establishment of Israel in 1948.
In the deadliest confrontation on the anniversary for years, Israeli forces shot protesters as they surged towards it frontiers with Syria, Lebanon and Gaza.
Israel’s leaders condemned the demonstrations, called for calm but vowed to defend its borders.
The worst unrest was on the Lebanese border but Gaza, the West bank and the Golan Heights were also scenes of violence.
Lebanon, Syria, Jordon and Gaza are all home to thousands of Palestinians who fled or were driven out when the state of Israel was created.
This years rise in Palestinian nationalism is thought to have been fuelled by the popular revolts of the “Arab Spring”.
Live gunfire met demonstrators as they crossed onto the disputed Golan Heights near Syria.
Syria which has been internationally criticised for its own crackdown on unrest has called Israel’s response “a criminal act”.