They have been given 24 hours to pack up their tents and leave.
The City of London has issued legal papers on anti-capitalist protesters outside St Paul’s Cathedral near the financial district.
Officials say they will take their case to the high Court if the initial eviction orders are ignored.
Some may not give up that easily, however.
One woman said: “We’re not here just because we like sleeping on the streets of London. I’d actually prefer to be at home in my own bed, but I feel compelled to be here because nobody’s speaking about the injustice that’s going on right around us. The corporation of London is a tax haven for multi-national criminals, as far as I can see.”
St Paul’s earlier dropped its own plans for legal action after the issue caused division within its ranks.
The eviction order in London comes after police in New York evicted protesters from a park near Wall Street on Tuesday.