The Croatian Serb rebel leader Goran Hadzic has denied charges of war crimes during the fighting in former Yugoslavia between 1991 and 1995.
He pleaded not guilty at the International Criminal Tribunal at the Hague in the Netherlands.
In his first appearance last month he had refused to enter a plea.
Hadzic was arrested in Serbia in July. He was the last of the country’s major war crimes suspects wanted by the Tribunal after the detention in May of Bosnian Serb General Ratko Mladic.
He proclaimed a Serbian republic within Croatia in the early nineties and appointed himself president.
He is accused of attempting to remove Croats and other non-Serbs from the territory and the extermination of hundreds more.
In one case prosecutors say he was responsible for the forced removal of 264 people from a hospital in 1991, who it is claimed were then detained and tortured before being executed at a remote site and buried in mass graves.